2 edition of Rat liver perfusion found in the catalog.
Rat liver perfusion
Terence Anthony Maguire
Written in English
Thesis (Ph. D.)--The Queen"s University of Belfast, 1984.
|The Physical Object|
The isolated perfused rat liver: standardization of a time-honoured model M Bessems 1, N A t Hart 2, R Tolba 3, B M Doorschodt 1, H G D Leuvenink 2, R J Ploeg 2, T Minor 3 and T M van Gulik 1 1 Surgical Laboratory, Academic Medical Center, Amsterdam, The Netherlands; 2 Surgical Research Laboratory, University Hospital Groningen, Groningen, The Netherlands; 3 Surgical Research Division, In conclusion, our laboratory‐scaled normothermic portovenous ex vivo liver perfusion system enables rat liver preservation for 6 hours. Both dialysis and glycine treatment were shown to be synergistic for preservation of the integrity of normal and DCD liver grafts. Citing Literature. Vol Issue 2.
Superimposed fluorescence traces of four anoxic cy cles in perfused rat liver (see ref. l). surface fluorescence of Hb-free perfused liver (prim. 36SmfjL; sec. #50mfi) CONTROL OF ENERGY METABOLISM Pour fluorescence traces have been recorded on the same area of liver surface, but at different times within three hours of :// The recent clinical application of perfusion technology for the machine preservation of donation after cardiac death (DCD) grafts has some advantages. Oxygenation has been proposed for the preservation of DCD liver grafts. The aim of this study is to clarify whether the use of HbV-containing preservation solution during the subnormothermic machine perfusion (SNMP) of the liver graft ?id=/
The perfusion system was evaluated for repeatability and sensitivity using isolated rat liver and exposed rat kidney tests. Perfusion in the isolated liver tests was varied by controlling the flow of the perfusate into the liver, and the perfusion in the exposed kidney tests was varied by temporarily occluding blood flow through the renal The reduction of oxidative stress is suggested to be one of the main mechanisms to explain the benefits of subnormothermic perfusion against ischemic liver damage. In this study we investigated the early cellular mechanisms induced in isolated rat livers after 15 min perfusion at temperatures ranging from normothermia (37°C) to subnormothermia (26°C and 22°C).
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In a rat liver perfusion system, more than 90% of C-menadione (14 C-VK 3) was metabolized within 5 h and 53%, 33%, and 15% was detected in the blood, bile, and liver, respectively . DT-diaphorase (NAD(P)H:quinone oxidoreductase) is at first involved in the metabolism of menadione, to reduce this to 1,4-dihydroxy-2 Perfusion values are estimated from the heat flux measurements using a mathematical model of the system with a minimization procedure.
The current research demonstrates the sensitivity and repeatability of the thermal blood perfusion system using two animal tissue models: an exposed rat kidney and an isolated rat :// Isolated rat liver perfusion.
On the th day after inoculation with tumor cells, the model and control rats were subjected to isolated liver perfusion.
The procedure for isolated liver perfusion was similar to that described in a previous publication. The perfusion conditions were in accordance with the standards proposed by Bessems. The Extracorporeal blood perfusion of the recellularized liver graft. We placed the recellularized liver graft in the extracorporeal blood perfusion system using a live rat to evaluate the antithrombotic ability of endothelial cells and the feasibility of our recellularized liver for blood :// Isolated hepatic perfusion (IHP) with melphalan is used for patients with nonresectable metastases confined to the liver.
To improve the efficacy of IHP and to reduce the toxicity to the liver, reversion (retrograde perfusion) of the bloodstream through the liver in a rat model was studied. For live liver perfusion depend upon an adequate supply ol ox-ygen.
This can be achieved by using either erythro-cyte-free perfusate at a flow rate greater than 6 mI/ min/g liver or a 20% erythrocyte-containing perfusate at 2 mI/min/g. ~ Academic Presa, Inc. INTRODUCTION The isolated perfused rat liver is a widely used DCD rat liver grafts did not differ in rat age and weight, liver weight, or cold ischemia time compared with normal livers, but they showed significantly impaired macroscopic flush (P = ; data not shown).
Perfusion pressure was significantly higher at the start of the perfusion An optimized model for rat liver perfusion studies. Journal of Surgical Research, 66 (), pp. Google Scholar. Compagnon et al., P. Compagnon, B. Clément, J.P. Campion, K. BoudjemaEffects of hypothermic machine perfusion on rat liver function depending on the route of :// Prashanth Sreeramoju, Steven K.
Libutti, in Advances in Genetics, III Isolated Liver Perfusion. Isolated hepatic perfusion (IHP) is a regional treatment technique that isolates the vasculature to the liver in order to allow the delivery of high-dose chemotherapy, biologic agents, and hyperthermia directly to unresectable cancers confined to the :// /liver-perfusion.
INTRODUCTION. Many modifications of the isolated perfused rat liver (IPRL) techniques have been described, but the majority of investigators have used variations of the original perfusion arrangement introduced by Brower and Miller [1–6].At our laboratories this organ perfusion technique is performed to assess the viability of preserved livers prior to :// Alternative liver preservation techniques.
Optimal preservation has been a major focus for as long as organ transplantation has been a reality. The first liver transplants were performed incorporating pump-driven perfusion of the organ in an attempt to maintain oxygenation of the liver relatively high complexity of these machine perfusion modalities resulted in a quick replacement by Objective.
To assess the application value of perfusion-weighted imaging (PWI) in early diagnosis, quantitation, and hepatic fibrosis staging by analyzing the related parameters in hepatic fibrosis. Methods.
A total of 60 rats were randomly divided into the hepatic fibrosis and control groups, and carbon tetrachloride (CCL4) was used to establish the liver fibrosis :// rat liver. These data also confirm that the IPRL modi fied for simultaneous perfusion of two livers is effi cient, and that with this technique the rat liver can be optimally perfused for up to 3 hr with oxygenated Krebs Henseleit buffer without additives (Group B) and without blood.
These two improvements should This is "Rat Liver Perfusion Surgey - First Cut" by Justin Morrison on Vimeo, the home for high quality videos and the people who love :// Background Normothermic ex vivo liver perfusion (NEVLP) is a promising strategy to increase the donor pool in liver transplantation.
Small animal models are essential to further investigate questions regarding organ preservation and reconditioning by NEVLP. A dual vessel small animal NEVLP (dNEVLP) model was developed using metamizole as a vasodilator and compared to conventional ?id=/ Abstract.
In aerobic conditions the isolated perfused liver from well-fed rats rapidly formed lactate from endogenous glycogen until the lactate concentration in the perfusion medium reached about 2m m (i.e.
the concentration of lactate in blood in vivo) and then production te was formed in proportion to the lactate, the [lactate]/[pyruvate] ratio remaining between 8 and | Extracorporeal blood perfusion of the recellularized liver graft We placed the recellularized liver graft in the extracorporeal blood perfusion system using a live rat to evaluate the antithrombotic abil-ity of endothelial cells and the feasibility of our recellularized liver for blood :// The introduction of extracorporeal liver perfusion systems in the clinical routine depends on feasibility.
Conceivably, perfusion could be performed during recipient preparation. We investigated whether a novel rat liver machine perfusion applied after in situ ischemia and cold storage can rescue NHBD liver :// Porcine and murine (rat) models of hypothermic, subnormothermic, and normothermic ex vivo liver perfusion have been described in the literature.
Although controversy still exists regarding perfusion temperature, it has been shown that that machine perfusion can improve function of liver grafts regardless of temperature :// Primary rat hepatocytes and LSECs were seeded into decellularized whole‐liver scaffolds via the biliary duct and portal vein, respectively.
Biliary duct seeding provided appropriate hepatocyte distribution into the parenchymal space, and portal vein–seeded LSECs simultaneously lined the portal lumen, thereby maintaining function and ://.
Table 1 shows the simultaneous retroviral titration performed in the inflow and outflow lines during the rat liver perfusion. Considering a liver passage of the perfusate at 30 and 60 min, the The DCD liver quality improved gradually with perfusion time, but liver function, lactate clearance, bile production, and histopathology deteriorated after 6-h perfusion, and DCD liver quality decreased, suggesting that the best and longest time for storing DCD liver in the rat NMP system was 6 h and that further perfusion might affect DCD Summary.
A technique for isolating portal fibroblasts from rat liver is described. Livers are perfused and digested in situ with collagenase, followed by ex vivo digestion of the liver slurry and size selection of cells. This method provides a pure population of portal fibroblasts without the need for passage in ://