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2 edition of Effect of low levels of x-radiation on the antigenicity of Escherichia Coli found in the catalog.

Effect of low levels of x-radiation on the antigenicity of Escherichia Coli

Gerald E. Longhurst

Effect of low levels of x-radiation on the antigenicity of Escherichia Coli

by Gerald E. Longhurst

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Published by University of Toronto, School of Graduate Studies in Toronto .
Written in English


Edition Notes

Thesis (M.Sc.D.)--University of Toronto, 1968.

StatementGerald E. Longhurst.
ID Numbers
Open LibraryOL17534297M

From international conference on the bases of the biological effects of ultraviolet radiation; Brno, Czechoslovakia (2 Oct Ethidium bromide (EB), an intercalating dye, was shown to be a potent inhibitor of the host cell reactivation of ultraviolet (uv)treated bacteriophage T/sub 1/, and of the dark repair of premutational damage produced by low dosages of uv-administered to Escherichia coli. @article{osti_, title = {AN AFTEREFFECT OF IONIZING RADIATION ON A MUTANT OF ESCHERICHIA COLI}, author = {Adler, H I}, abstractNote = {A study- is being made of the effect of ionizing radiation on a mutant of Escherichia coli Monod that requires hemin for the synthesis of porphyrin- containing emymes. If growth occurs in the absence of hemin and the cells are .

UV Radiation A UV disinfection system consists of mercury arc lamps, a contact vessel, and ballasts. The source of UV radiation is either a low- or a medium-pressure mercury arc lamp with low or high intensity. Medium- pressure lamps are generally used for large facilities. The optimum wavelength to effectively inactivate microorganisms is in the. Inhibitory effects of radiolytic products were studied using Escherichia coli, Pediococcus cerevisiae, and two radiation-resistant microorganisms, an isolate of Moraxella-Acinetobacter and a Micrococcus sp. End Products of an irradiation dose of Krads completely inhibited resistant organisms on an experimental medium with a very low concentration of nutrients.

The hypothesized high-frequency initiating event could conceivably be a change in gene expression (for example, see Fa80) of a type that might occur in a large proportion of irradiated cells (Sc85); in Escherichia coli, for example, radiation induces an error-prone DNA repair system (the SOS system) which leads to mutations that would otherwise. Firstly, our research group identified Sutai pigs’ phenotypes that exhibited extreme resistance and susceptibility to the Escherichia coli F18 respectively, and then eight ETEC (Enterotoxigenic Escherichia coli) Fresistant piglets and eight ETEC Fsensitive piglets were selected. Then, the TAP1 (Transporter associated with antigen processing) mRNA relative expression levels were.


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Effect of low levels of x-radiation on the antigenicity of Escherichia Coli by Gerald E. Longhurst Download PDF EPUB FB2

The dose-response of radiation-induced PC in D. radiodurans lags by a factor of 21 for ionizing radiation and by a factor of 25 for UVC light relative to that observed in E. coli, suggesting the operation of distinct mechanisms of PC and/or differences in proteome protection against radiation-induced exposure to high levels of ionizing radiation and UVC, the similar PC saturation Cited by: Effect of low level X radiation on the antigenicity of Escherichia coli.

Longhurst GE, Goldner M, Clark GM, Poyton HG. PMID: [PubMed - indexed for MEDLINE] MeSH Terms. Antigen-Antibody Reactions/radiation effects* Escherichia coli/immunology* Escherichia coli/radiation effects* Immunodiffusion* Immunoelectrophoresis* Radiation Effects*Cited by: 1.

The present study investigated the effects of microwave (MW) radiation applied under a sublethal temperature on Escherichia experiments were conducted at a frequency of 18 GHz and at a temperature below 40°C to avoid the thermal degradation of bacterial cells during by:   The doses needed to reduce E.

coli OH7, L. monocytogenes, S. enterica and S. flexneri, by 5 log reduction, on the surface of Roma tomatoes were less than those needed on other d () found that more than a 5 log CFU reduction of E. coli OH7, L. monocytogenes, S. enterica and S. flexneri was achieved with kGy X-ray on shredded iceberg Cited by:   The main goal of this investigation was to study the efficacy of X-ray doses (,and kGy) on inoculated Escherichia coli O H7, Listeria monocytogenes, Salmonella enterica and Shigella flexneri on shredded iceberg lettuce.

The second goal was to study the effect of X-ray on the inherent microflora counts and visual color of shredded iceberg lettuce during Cited by: Rentschler HC, Nagy R, Mouromseff G. Bactericidal Effect of Ultraviolet Radiation. J Bacteriol. Jun; 41 (6)– [PMC free article] Roepke RR, Libby RL, Small MH.

Mutation or Variation of Escherichia coli with Respect to Growth Requirements. J Bacteriol. Oct; 48. The present study investigated the effects of microwave (MW) radiation on Escherichia coli applied under a sub-lethal temperature. The experiments were conducted at a frequency of 18 GHz and performed at a temperature below 40oC to avoid the thermal degradation of bacterial cells during exposure.

The absorbed power was calculated to be kW/m3 and the electric field was. Accordingly, it does not support the notion of radiation hormesis, that hypothesises low levels of radiation may be stimulatory or even beneficial for cells by stimulating the activation of repair. Ozonated water has been used as a strong antimicrobial agent against foodborne pathogens.

In this study, the combined effect of low level ozonated water and different added components, including % starch and metal ions (1 mM CuCl22H2O and mM AgNO3), on inactivation of Escherichia coli OH7 and Listeria monocytogenes was investigated.

This study evaluated the efficacy of gamma irradiation to inactivate food‐borne pathogens on pistachios (Pistacia vera L.).Pistachios inoculated with Escherichia coli OH7, Salmonella Typhimurium and Listeria monocytogenes were subjected to gamma irradiation in the range of 0, 05, 1, 3 and 5 kGy, and colour change was evaluated after treatment and during storage at room temperature.

The ground pork was radiation-sterilized, mixed with the ingredients, and inoculated by E. coli, and irradiated (, 1, 2, 3, and 5 kGy) in order to obtain the effective D 10 values and relative radiation sensitivity (RRS; Table 1).All food ingredients tested were effective in improving the radiation inactivation efficiency of E.

coli, resulting in 2–4 times lower D 10 values when compared. We have generated extreme ionizing radiation resistance in a relatively sensitive bacterial species, Escherichia coli, by directed evolution. Four populations of Escherichia coli K were derived independently from strain MG, with each specifically adapted to survive exposure to high doses of ionizing radiation.

D37 values for strains isolated from two of the populations approached that. Direct inoculation into media containing bile salts and antibiotics is unsuitable for the detection of acid/ salt stressed Escherichia coli OH7.

Lett. Appl. Microbiol. [9] Weagent, S.D., J.L. Bryant, and K.G. Jinneman. An improved rapid technique for isolation of Escherichia coli OH7 from foods. Food Prot. BILLEN D, LICHSTEIN HC.

The effect of x radiation on the adaptive formation of formic hydrogenase in Escherichia coli. J Bacteriol. Apr; 63 (4)– [PMC free article] BRANDT CL, FREEMAN PJ, SWENSON PA. The effect of radiations on galactozymase.

The effects of fat level and low fat formulation on survival of Escherichia coli OH7 isolate P heated in ground beef [7%, 10% and 20% fat], pork sausage [7%, 10%, and 30% fat], chicken (3% and 11% fat), and turkey (3% and 11% fat) were determined by D‐ and z‐values.

D‐values for E. coli H7 in lowest fat products were lower than in traditional beef and pork products (P. This study was conducted to evaluate the inactivation effect of X-ray treatments on Escherichia coli O H7, Salmonella enteric (S.

enterica), Shigella flexneri (S. flexneri) and Vibrio parahaemolyticus (V. parahaemolyticus) artificially inoculated in ready-to-eat (RTE) shrimp.A mixed culture of three strains of each tested pathogen was used to inoculate RTE shrimp.

Escherichia coli is one of the most frequently diagnosed causes of bovine mastitis, and is typically associated with acute, clinical mastitis. The objective of the present study was to evaluate the long term effects of intramammary infections by E.

coli on milk yield and quality, especially milk coagulation. Twenty-four Israeli Holstein cows diagnosed with clinical mastitis due to. Investigating the Effects of Time and Temperature on the Growth of Escherichia coli OH7 and Listeria monocytogenes in Raw Cow’s Milk Based on Simulated Consumer Food Handling Practices by Roselyn M.

Leclair 1,*, Sarah K. McLean 1, Louise A. Dunn 1, Denny Meyer 2 and Enzo A. Palombo 1. Inactivated vaccines are commonly produced by incubating pathogens with chemicals such as formaldehyde or β-propiolactone.

This is a time-consuming process, the inactivation efficiency displays high variability and extensive downstream procedures are often required. Moreover, application of chemicals alters the antigenic components of the viruses or bacteria, resulting in reduced antibody. Inactivated vaccines are commonly produced by incubating pathogens with chemicals such as formaldehyde or β-propiolactone.

This is a time-consuming process, the inactivation efficiency displays high variability and extensive downstream procedures are often required. Moreover, application of. Abstract: This study evaluated the effect of produce type, resuspension medium, dose uniformity ratio (DUR), and sample preparation conditions (tissue exposure, MAP, anoxia) on the D 10 ‐value of an Escherichia coli cocktail (BAA‐, BAA‐, and BAA‐) and Salmonella Typhimurium LT2 inoculated on the surfaces of tomato, cantaloupe, romaine lettuce, and baby spinach.WITH the exception of oxygen and nitric oxide, chemical agents which enhance the effect of radiation on bacteria have received little attention.

In the experiments to be described, N.In our previous work, we designed and implemented a synthetic metabolic pathway for 1,2,3-trichloropropane (TCP) biodegradation in Escherichia coli.

Significant effects of metabolic burden and toxicity exacerbation were observed on single cell and population levels. Deeper understanding of mechanisms underlying these effects is extremely important for metabolic engineering of efficient.